In order to better reduce lake eutrophication, based on five ecoregions of lakes, the relationships of total nitrogen (TN), total phosphorus (TP), and TN/TP ratio with chlorophyll-a (Chl-a) in 100 lakes were discussed, furthermore, strategies of nutrient control were proposed respectively. Results showed that among the five ecoregions, the lake eutrophication level was the lowest in Dongbei ecoregion and the highest in Huabei ecoregion, but the eutrophication level in lakes of all the five ecoregions is increasing in recent years. Algal growth in lakes of Dongbei and Huabei ecoregions was limited by phosphorus, while it was simultaneously limited by nitogen and phosphorus in lakes of other three ecoregions (Zhongdongbu, Yungui and Mengxin). In lakes with TN/TP < 10, significant correlation between Chl-a concentration and TN was found in lakes of the five ecoregions except for Huabei ecoregion, and significant correlation between Chl-a concentration and TP was found in lakes of Dongbei and Mengxin ecoregions. In lakes with TN/TP > 17, significant correlation between Chl-a concentration and TP was found in lakes of the five ecoregions except for Mengxin ecoregion, and significant correlation between Chl-a concentration and TN was found in lakes of Zhongdongbu, Yungui and Mengxin ecoregions. In lakes with 10 < TN/TP < 17, no significant correlation between Chl-a and TN (or TP) was found in lakes of all ecoregions except for Zhongdongbu ecoregion where Chl-a concentration was significantly affected by TN. As for strategies of nutrient control and reduction in the five ecoregions, lakes of Huabei ecoregion should adopt TP control in priority, and in Dongbei ecoregion, TP and TN should be controlled simultaneously in lakes with TN/TP < 10, while other lakes should focus on TP control. Lakes in other three ecoregions (Zhongdongbu, Mengxin and Yungui) should control TP and TN simultaneously.