Imaging of mitochondrial and nuclear Ca2+ dynamics in Arabidopsis roots

Cold Spring Harb Protoc. 2013 Aug 1;2013(8):781-5. doi: 10.1101/pdb.prot073049.

Abstract

Here we report a method for analyzing mitochondrial Ca(2+) dynamics in plant root cells by means of the newly generated cameleon probe 4mt-YC3.6. The use of plants expressing both nuclear- and mitochondrial-targeted cameleon, along with the resolution of confocal laser scanning microscopy (CLSM), allow simultaneous recordings of mitochondrial and nuclear Ca(2+) dynamics within the same cell.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / cytology
  • Calcium / analysis*
  • Calcium-Binding Proteins / genetics
  • Calcium-Binding Proteins / metabolism*
  • Cations, Divalent / analysis
  • Cell Nucleus / chemistry*
  • Cytological Techniques / methods*
  • Fluorescent Dyes / metabolism*
  • Microscopy, Confocal / methods*
  • Mitochondria / chemistry*
  • Plant Roots / cytology
  • Staining and Labeling / methods
  • Transgenes

Substances

  • Calcium-Binding Proteins
  • Cations, Divalent
  • Fluorescent Dyes
  • yellow cameleon
  • Calcium