Abstract
Here we report a method for analyzing mitochondrial Ca(2+) dynamics in plant root cells by means of the newly generated cameleon probe 4mt-YC3.6. The use of plants expressing both nuclear- and mitochondrial-targeted cameleon, along with the resolution of confocal laser scanning microscopy (CLSM), allow simultaneous recordings of mitochondrial and nuclear Ca(2+) dynamics within the same cell.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arabidopsis / cytology
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Calcium / analysis*
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Calcium-Binding Proteins / genetics
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Calcium-Binding Proteins / metabolism*
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Cations, Divalent / analysis
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Cell Nucleus / chemistry*
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Cytological Techniques / methods*
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Fluorescent Dyes / metabolism*
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Microscopy, Confocal / methods*
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Mitochondria / chemistry*
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Plant Roots / cytology
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Staining and Labeling / methods
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Transgenes
Substances
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Calcium-Binding Proteins
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Cations, Divalent
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Fluorescent Dyes
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yellow cameleon
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Calcium