Cholinesterase (ChE) activity has been used for many years as a biomarker of exposure to organophosphate and carbamate pesticides. Recent studies have demonstrated that there could be biological factors that determine ChE type and levels; thus, juvenile Sergeant major (Abudefduf saxatilis) ChE enzymes were biochemically characterized. ChE enzymes found in the head and trunk were evaluated for their substrate preference and sensitivity to selective inhibitors. The use of the head and trunk was chosen as a strategy to reduce dissection time and to ensure sample uniformity between stations. The results indicated that there are two types of ChE enzymes in the head: acetylcholinesterase (AChE) and atypical butyrylcholinesterase (BChE) that exhibits intermediate characteristics of human AChE and BChE activities. Atypical BChE is predominantly found in the trunk. The results also indicated that the ChE activity found in A. saxatilis may be used as a biomarker in studies monitoring the Mexican Caribbean.
Keywords: 1,5-bis-(4-allyldimethyl-ammoniumphenyl)-pentan-3-one dibromide; 5,5-dithiobis(2-nitrobenzoic acid); AChE; ASChI; Abudefduf saxatilis; Acetylcholinesterase; Acetylthiocholine iodide; BChE; BSChI; BW284c51; Butyrylcholinesterase; Butyrylthiocholine iodide; ChE; Cholinesterase; DTNB; Damselfish; Iso-OMPA; Tetraisopropyl pyrophosphoramide.
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