Herpesvirus 6 glycoproteins B (gB), gH, gL, and gQ are necessary and sufficient for cell-to-cell fusion

J Virol. 2013 Oct;87(19):10900-3. doi: 10.1128/JVI.01427-13. Epub 2013 Jul 24.

Abstract

The human herpesvirus 6 (HHV-6) envelope glycoprotein gH/gL/gQ1/gQ2 complex associates with host cell CD46 as its cellular receptor. Although gB has been suggested to be involved in HHV-6 infection, its function in membrane fusion has remained unclear. Here, we have developed an HHV-6A (strain GS)and HHV-6B (strain Z29) virus-free cell-to-cell fusion assay and demonstrate that gB and the gH/gL/gQ1/gQ2 complex are the minimum components required for membrane fusion by HHV-6.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Fusion*
  • Cells, Cultured
  • DNA, Viral / genetics
  • Flow Cytometry
  • Glycoproteins / classification
  • Glycoproteins / genetics
  • Glycoproteins / metabolism*
  • Herpesviridae Infections / genetics
  • Herpesviridae Infections / metabolism*
  • Herpesviridae Infections / virology
  • Herpesvirus 6, Human / physiology*
  • Humans
  • Kidney / cytology
  • Kidney / metabolism
  • Kidney / virology
  • Luciferases / metabolism
  • Membrane Cofactor Protein / genetics
  • Membrane Cofactor Protein / metabolism*
  • Membrane Fusion*
  • Polymerase Chain Reaction

Substances

  • DNA, Viral
  • Glycoproteins
  • Membrane Cofactor Protein
  • Luciferases