Aptamer-based label-free detection of PDGF using ruthenium(II) complex as luminescent probe

E Babu; S Singaravadivel; P Manojkumar; S Krishnasamy; G Gnana kumar; S Rajagopal

doi:10.1007/s00216-013-7118-4

Aptamer-based label-free detection of PDGF using ruthenium(II) complex as luminescent probe

Anal Bioanal Chem. 2013 Aug;405(21):6891-5. doi: 10.1007/s00216-013-7118-4. Epub 2013 Jul 24.

Authors

E Babu¹, S Singaravadivel, P Manojkumar, S Krishnasamy, G Gnana kumar, S Rajagopal

Affiliation

¹ Department of Physical Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, 625 021, India.

PMID: 23881364
DOI: 10.1007/s00216-013-7118-4

Abstract

We report a simple, cost-effective, and label-free detection method, consisting of a platelet-derived growth factor (PDGF) binding aptamer and hydrophobic Ru(II) complex as a sensor system for PDGF. The binding of PDGF with the aptamer results in the weakening of the aptamer-Ru(II) complex, monitored by luminescence signal. A substantial enhancement in the luminescence intensity of Ru(II) complex is observed in the presence of aptamer due to the hydrophobic interaction. Upon addition of PDGF, the luminescence intensity is decreased, due to the stronger interaction between the aptamer and PDGF resulting in the displacement of Ru(II) complex to the aqueous solution. Our assay can detect a target specifically in a complex medium such as the mixture of proteins, at a concentration of 0.8 pM.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aptamers, Nucleotide / chemistry*
Luminescent Measurements / methods*
Molecular Probe Techniques*
Molecular Probes / analysis
Molecular Probes / chemistry
Platelet-Derived Growth Factor / analysis*
Platelet-Derived Growth Factor / chemistry
Protein Interaction Mapping / methods*
Ruthenium / analysis*
Ruthenium / chemistry*
Staining and Labeling

Substances

Aptamers, Nucleotide
Molecular Probes
Platelet-Derived Growth Factor
Ruthenium