Spectral and redox properties of the GFP synthetic chromophores as a function of pH in buffered media

Joseph E Reynolds 3rd; Mira Josowicz; Phillip Tyler; Russell B Vegh; Kyril M Solntsev

doi:10.1039/c3cc44113j

Spectral and redox properties of the GFP synthetic chromophores as a function of pH in buffered media

Chem Commun (Camb). 2013 Sep 14;49(71):7788-90. doi: 10.1039/c3cc44113j.

Authors

Joseph E Reynolds 3rd¹, Mira Josowicz, Phillip Tyler, Russell B Vegh, Kyril M Solntsev

Affiliation

¹ School of Chemistry and Biochemistry, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, GA 30332-0400, USA.

PMID: 23880780
DOI: 10.1039/c3cc44113j

Abstract

The effect of pH on the spectral and redox properties of model GFP synthetic chromophores (Cros) in a universal buffer system was investigated. The Cro hydroxy-derivatives demonstrated a Nernst-type electrochemical dependence of the anodic potential within the pH 2-8 range. Analogous studies on various fluorescent proteins were unsuccessful.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Buffers
Electrons
Green Fluorescent Proteins / chemistry*
Green Fluorescent Proteins / metabolism
Hydrogen-Ion Concentration
Oxidation-Reduction
Water / chemistry

Substances

Buffers
Water
Green Fluorescent Proteins