Rapid genotyping of three polymorphisms in the LBP gene using a triplex pyrosequencing approach

Ling Zeng; Jing Yu; Wei Gu; An-qiang Zhang; Cai-li Yue; Ke-hong Chen; Jian-xin Jiang

doi:10.1016/j.mimet.2013.07.008

Rapid genotyping of three polymorphisms in the LBP gene using a triplex pyrosequencing approach

J Microbiol Methods. 2013 Sep;94(3):300-2. doi: 10.1016/j.mimet.2013.07.008. Epub 2013 Jul 20.

Authors

Ling Zeng¹, Jing Yu, Wei Gu, An-qiang Zhang, Cai-li Yue, Ke-hong Chen, Jian-xin Jiang

Affiliation

¹ State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, China.

PMID: 23876431
DOI: 10.1016/j.mimet.2013.07.008

Abstract

We described a triplex polymerase chain reaction (PCR) and triplex pyrosequencing assay which allowed a simultaneous determination of three tag single nuleotide polymorphisms (tag SNPs) in the lipopolysaccharide-binding protein (LBP) gene: rs1780623, rs11536972 and rs2232618. This method enables a fast and cost-effective genotyping and a simultaneous determination of the three tag SNPs.

Keywords: Lipopolysaccharide-Binding Protein; Single Nuleotide Polymorphism; Triplex pyrosequencing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acute-Phase Proteins / genetics*
Carrier Proteins / genetics*
Genotyping Techniques / methods*
Humans
Membrane Glycoproteins / genetics*
Polymorphism, Single Nucleotide / genetics*
Sequence Analysis, DNA / methods*

Substances

Acute-Phase Proteins
Carrier Proteins
Membrane Glycoproteins
lipopolysaccharide-binding protein