Silicon dioxide thin film mediated single cell nucleic acid isolation

PLoS One. 2013 Jul 10;8(7):e68280. doi: 10.1371/journal.pone.0068280. Print 2013.

Abstract

A limited amount of DNA extracted from single cells, and the development of single cell diagnostics make it necessary to create a new highly effective method for the single cells nucleic acids isolation. In this paper, we propose the DNA isolation method from biomaterials with limited DNA quantity in sample, and from samples with degradable DNA based on the use of solid-phase adsorbent silicon dioxide nanofilm deposited on the inner surface of PCR tube.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA / isolation & purification
  • Disposable Equipment
  • Female
  • Humans
  • Membranes, Artificial*
  • Nucleic Acids / isolation & purification*
  • Oocytes / metabolism
  • Plastics / chemistry
  • Polymerase Chain Reaction* / instrumentation
  • Polymerase Chain Reaction* / methods
  • Polypropylenes / chemistry
  • Rats
  • Silicon Dioxide / chemistry*
  • Single-Cell Analysis* / instrumentation
  • Single-Cell Analysis* / methods

Substances

  • Membranes, Artificial
  • Nucleic Acids
  • Plastics
  • Polypropylenes
  • Silicon Dioxide
  • DNA

Grants and funding

This work was supported by The Ministry of Education and Science of the Russian Federation (grants: 16.552.12.2006, 14.512.11.0036 and 14.132.21.1771); the President of Russian Federation Scholarship for Young Researchers and Graduate Students (to E.B. and M.P.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.