LC-ESI-MS/MS analysis and pharmacokinetics of jolkinolide B, a potential antitumor active component isolated from Euphorbia fischeriana, in rat plasma

Biomed Chromatogr. 2014 Feb;28(2):193-6. doi: 10.1002/bmc.3000. Epub 2013 Jul 19.

Abstract

A simple, specific and reproducible liquid chromatography-electrospray ionization mass spectrometry was developed and validated for the determination of jolkinolide B, a potential antitumor active component isolated from Euphorbia fischeriana, in rat plasma. Chromatographic separation was achieved on a Venusil MP-C18 column using an isocratic elution. Jolkinolide B and osthole (internal standard) were monitored by positive electrospray ionization in the selected reaction monitoring mode. Good linearity (r(2) > 0.996) was achieved by a weighted (1/x(2) ) linear least-squares regression over a concentration range of 6.50-2600 ng/mL. The accuracy and precision of the assay were satisfactory and the method proved to be applicable to pharmacokinetics following a single intravenous bolus injection of jolkinolide B to rats.

Keywords: LC-ESI-MS/MS; antitumor component; jolkinolide B; pharmacokinetics; rat plasma.

MeSH terms

  • Animals
  • Antineoplastic Agents / blood*
  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / pharmacokinetics*
  • Chromatography, Liquid / methods
  • Diterpenes / blood*
  • Diterpenes / chemistry
  • Diterpenes / pharmacokinetics*
  • Drug Stability
  • Euphorbia / chemistry*
  • Least-Squares Analysis
  • Male
  • Plant Extracts / chemistry*
  • Rats
  • Rats, Wistar
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spectrometry, Mass, Electrospray Ionization / methods
  • Tandem Mass Spectrometry / methods

Substances

  • Antineoplastic Agents
  • Diterpenes
  • Plant Extracts
  • jolkinolide B