Interleukin-10 (IL-10) is an important immunomodulatory cytokine that plays an obligate role in regulating inflammatory responses. Here we demonstrated the role of IL-10 in regulating crypts length and breadth as well as maintaining the survival of epithelial cells using rhesus colon explant cultures. Anti-IL-10 antibody treatment of colon explant cultures induced increased production of inflammatory cytokines/molecules like IFNγ, TNFα, CD107a and perforin as well as increased epithelial cell apoptosis compared to media controls tested. Our results suggest that IL-10 plays a crucial role in maintaining mucosal homeostasis by regulating mucosal IFNγ and TNFα cytokine production.
Keywords: AC-3; Apoptosis; Colon; Cytokine; EC; Epithelial cell; HIV; IL-10; JAK1; MAb; RM; SCID; SIV; T-helper 1; Th1; Tyk2; active caspase-3; epithelial cells; human immunodeficiency virus; interleukin-10; janus kinase 1; monoclonal antibody; rIL-10; recombinant IL-10; rhesus macaque; severe combined immunodeficiency; simian immunodeficiency virus; tyrosine kinase 2.
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