Gram-scale production of a basidiomycetous laccase in Aspergillus niger

J Biosci Bioeng. 2014 Jan;117(1):25-7. doi: 10.1016/j.jbiosc.2013.06.013. Epub 2013 Jul 16.

Abstract

We report on the expression in Aspergillus niger of a laccase gene we used to produce variants in Saccharomyces cerevisiae. Grams of recombinant enzyme can be easily obtained. This highlights the potential of combining this generic laccase sequence to the yeast and fungal expression systems for large-scale productions of variants.

Keywords: Aspergillus niger; Bioreactor; Glycosylation; Multicopper enzyme; Saccharomyces cerevisiae.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspergillus niger / enzymology*
  • Aspergillus niger / genetics
  • Electrophoresis, Polyacrylamide Gel
  • Hydrogen-Ion Concentration
  • Laccase / biosynthesis*
  • Laccase / genetics
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism*
  • Saccharomyces cerevisiae / metabolism

Substances

  • Recombinant Proteins
  • Laccase