A SEM and non-contact surface white light profilometry in vivo study of the effect of a crème containing CPP-ACP and fluoride on young etched enamel

Scanning. 2014 Mar-Apr;36(2):270-7. doi: 10.1002/sca.21102. Epub 2013 Jul 10.

Abstract

The aim of this in vivo study was to evaluate the short and a longer term effect on enamel of the application of a crème containing 10% CPP-ACP and 900 ppm fluoride, in orthodontically planned, high caries-risk patients. Epoxy resin replicas of upper lateral incisors were obtained from polyvinyl siloxane (PVS) impressions, before and after etching. The right incisors were left untreated in order to control saliva remineralizing potential. The upper left surfaces were coated with a pea-size amount of the crème. Replicas were obtained at 3 weeks and 6 months and analyzed by SEM and non-contact surface white light profilometry. In the treated sample the profilometric roughness parameters at 3 weeks were statistically significantly lower than the control group values (p < 0.05). At 3 weeks SEM images of the enamel surface showed fewer irregularities. After 6 months, differences between test and control groups were not present on SEM images and profilometric values. CPP-ACP and fluoride crème had positive in vivo effects on enamel surfaces. Significant differences in surface roughness existed after a 3-week period of crème use.

Keywords: SEM; dentistry; microstructure; replicas; surface analisys.

MeSH terms

  • Caseins / metabolism*
  • Dental Enamel / drug effects*
  • Dental Enamel / ultrastructure
  • Fluorides / metabolism*
  • Humans
  • Incisor / drug effects
  • Incisor / ultrastructure
  • Microscopy, Electron, Scanning
  • Surface Properties / drug effects*
  • Treatment Outcome

Substances

  • Caseins
  • casein phosphopeptide-amorphous calcium phosphate nanocomplex
  • Fluorides