Abstract
All together: Lipidated LC3 has been synthesized by expressed protein ligation. A TEV-cleavable MBP tag was employed to facilitate ligation under folding conditions and to solubilize the lipidated protein. The synthetic LC3-phosphatidylethanolamine (PE) mediates membrane tethering and fusion at the physiological concentration of PE, and could be a useful tool for autophagy studies.
Keywords:
LC3; autophagy; expressed protein ligation; lipidated proteins; membrane fusion; protein synthesis.
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Cysteine Endopeptidases / metabolism
-
Endopeptidases / metabolism
-
Humans
-
Light
-
Liposomes / metabolism*
-
Maltose-Binding Proteins / chemistry
-
Maltose-Binding Proteins / genetics
-
Maltose-Binding Proteins / metabolism
-
Microtubule-Associated Proteins / chemistry
-
Microtubule-Associated Proteins / genetics
-
Microtubule-Associated Proteins / metabolism*
-
Phosphatidylethanolamines / chemistry*
-
Recombinant Fusion Proteins / biosynthesis
-
Recombinant Fusion Proteins / chemistry
-
Recombinant Fusion Proteins / genetics
-
Scattering, Radiation
Substances
-
Liposomes
-
MAP1LC3A protein, human
-
Maltose-Binding Proteins
-
Microtubule-Associated Proteins
-
Phosphatidylethanolamines
-
Recombinant Fusion Proteins
-
phosphatidylethanolamine
-
Endopeptidases
-
TEV protease
-
Cysteine Endopeptidases