A new thiophenyl pyrazoline probe for Cu(2+) in aqueous solution was synthesized and characterized by IR, NMR, HRMS and X-ray analysis. The probe displays remarkably high selectivity and sensitivity for Cu(2+) with a detection limit of 1.919 × 10(-7) M in aqueous solution (EtOH:HEPES = 1:1, v/v, 0.02 M, pH = 7.2). In addition, the probe is further successfully used to image Cu(2+) in living cells and the probe possesses good reversibility.