Abstract
Here we report the presence and expression levels of the vanC1 and vanC(2/3) genes in vancomycin-susceptible strains of Enterococcus faecalis. The vanC1 and vanC(2/3) genes were located in the plasmid DNA and on the chromosome, respectively. Specific mRNA of the vanC1 gene was detected in one of these strains. Additionally, analysis of the vanC gene sequences showed that these genes are related to the vanC genes of Enterococcus gallinarum and Enterococcus casseliflavus. The presence of vanC genes is useful for the identification of E. gallinarum and E. casseliflavus. Moreover, this is the first report of vanC mRNA in E. faecalis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Bacterial Agents / pharmacology*
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Bacterial Proteins / genetics*
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Chickens
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Cloaca / microbiology
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DNA, Bacterial / analysis
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Disk Diffusion Antimicrobial Tests
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Enterococcus faecalis / drug effects*
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Enterococcus faecalis / genetics*
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Enterococcus faecalis / isolation & purification
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Genes, Bacterial / genetics
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Real-Time Polymerase Chain Reaction
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Vancomycin / pharmacology*
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Vancomycin Resistance / genetics*
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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DNA, Bacterial
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VanC protein, Bacteria
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Vancomycin
Grants and funding
Financial support: CNPq (470291/2010-9), CAPES ACF, AMLR, JF and PAd’A are CNPq research fellows.