Protein-binding aptamer assisted signal amplification for the detection of influenza A (H1N1) DNA sequences based on quantum dot fluorescence polarization analysis

Analyst. 2013 Sep 7;138(17):4722-7. doi: 10.1039/c3an00830d. Epub 2013 Jul 3.

Abstract

We report a fluorescence polarization platform for H1N1 detection based on the construction of a DNA functional QD fluorescence polarization probe and a bi-functional protein binding aptamer (Apt-DNA). The assay has a linear range from 10 nM to 100 nM with a detection limit of 3.45 nM and is selective over the mismatched bases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aptamers, Nucleotide / chemistry*
  • Aptamers, Nucleotide / metabolism*
  • DNA, Viral / analysis*
  • DNA, Viral / genetics
  • Fluorescence Polarization
  • Fluorescent Dyes / chemistry
  • Fluorescent Dyes / metabolism*
  • Hydrogen-Ion Concentration
  • Influenza A Virus, H1N1 Subtype / genetics*
  • Influenza A Virus, H1N1 Subtype / isolation & purification
  • Nucleic Acid Hybridization
  • Quantum Dots*
  • Streptavidin / metabolism*

Substances

  • Aptamers, Nucleotide
  • DNA, Viral
  • Fluorescent Dyes
  • Streptavidin