Production and characterization of humanized Rag2-/-γc -/- mice

Freddy M Sanchez; German I Cuadra; Stanton J Nielsen; Anne Tanner; Bradford K Berges

doi:10.1007/978-1-62703-481-4_2

Production and characterization of humanized Rag2-/-γc -/- mice

Methods Mol Biol. 2013:1031:19-26. doi: 10.1007/978-1-62703-481-4_2.

Authors

Freddy M Sanchez¹, German I Cuadra, Stanton J Nielsen, Anne Tanner, Bradford K Berges

Affiliation

¹ Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, USA.

PMID: 23824882
DOI: 10.1007/978-1-62703-481-4_2

Abstract

Mice reconstituted with human immune cells represent a model to study the development and functionality of the human immune system. Recent improvements in humanized mice have resulted in multi-lineage hematopoiesis, prolonged human cell engraftment that is detectable in many mouse organs, and the ability to generate de novo human innate and adaptive immune responses. Here, we describe the methods used to produce and characterize humanized Rag2(-/-)γc(-/-) mice.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Monoclonal, Humanized
DNA-Binding Proteins / genetics*
DNA-Binding Proteins / immunology
Disease Models, Animal
Hematopoiesis / immunology
Humans
Immune System*
Mice
Mice, Transgenic / genetics*
Nuclear Proteins / genetics*
Nuclear Proteins / immunology
Transplantation, Heterologous / methods*

Substances

Antibodies, Monoclonal, Humanized
DNA-Binding Proteins
Nuclear Proteins
RAG2 protein, human