Evaluation of a new commercially available immunoglobulin M capture enzyme-linked immunosorbent assay for diagnosis of dengue virus infection

Madhuri Namekar; Esther M Ellis; Maile O'Connell; Joe Elm; Alexandra Gurary; Sarah Y Park; Allison Imrie; Vivek R Nerurkar

doi:10.1128/JCM.00351-13

Evaluation of a new commercially available immunoglobulin M capture enzyme-linked immunosorbent assay for diagnosis of dengue virus infection

J Clin Microbiol. 2013 Sep;51(9):3102-6. doi: 10.1128/JCM.00351-13. Epub 2013 Jul 3.

Authors

Madhuri Namekar¹, Esther M Ellis, Maile O'Connell, Joe Elm, Alexandra Gurary, Sarah Y Park, Allison Imrie, Vivek R Nerurkar

Affiliation

¹ Department of Tropical Medicine, Medical Microbiology and Pharmacology, John A Burns School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii, USA.

Abstract

We evaluated the FDA-cleared InBios dengue virus (DENV) IgM capture enzyme-linked immunosorbent assay (ELISA) for qualitative detection of anti-DENV IgM antibodies from 79 serum samples obtained from dengue virus-infected patients or suspected dengue cases. The agreement, sensitivity, and specificity of the InBios assay compared to the gold standard in-house DENV IgM capture ELISA were 94, 92, and 94%, respectively. We conclude that the InBios DENV IgM capture ELISA can be effectively used for rapid diagnosis of acute or recent DENV infection.

Publication types

Comparative Study
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Antibodies, Viral / blood*
Clinical Laboratory Techniques / methods*
Dengue / diagnosis*
Dengue Virus / immunology*
Enzyme-Linked Immunosorbent Assay / methods
Humans
Immunoglobulin M / blood*
Sensitivity and Specificity

Substances

Antibodies, Viral
Immunoglobulin M

Abstract

Publication types

MeSH terms

Substances

Grants and funding