Content of glycogen and activity of hexokinase (HK) were similar to control value, while activity of glucose-6-phosphate dehydrogenase (G6PD) was increased in liver tissue of rats after 20 days long hypokinesia. Acute stress (or readaptation stress) was observed in the animals after discontinuation of the immobilization, which was characterized by a decrease in content of glycogen and in activity of HK and G6PD in liver tissue. These patterns studied exceeded the control values within the first week after immobilization, they became similar to controls within 10 days and decreased repeatedly at the period between 20 and 30 days. Only within 45-65 days of the readaptation period content of glycogen as well as activity of HK and G6PD were completely normalized.