Metabolomic Analysis of the Effect of Postnatal Hypoxia on the Retina in a Newly Born Piglet Model

PLoS One. 2013 Jun 18;8(6):e66540. doi: 10.1371/journal.pone.0066540. Print 2013.

Abstract

The availability of reliable biomarkers of brain injury secondary to birth asphyxia could substantially improve clinical grading, therapeutic intervention strategies, and prognosis. In this study, changes in the metabolome of retinal tissue caused by profound hypoxia in an established neonatal piglet model were investigated using an ultra performance liquid chromatography - quadrupole time of flight mass spectrometry (UPLC-QTOFMS) untargeted metabolomic approach, which included Partial Least Squares - Discriminant Analysis (PLSDA) multivariate data analysis. The initial identification of a set of discriminant metabolites from UPLC-QTOFMS data was confirmed by target UPLC-MS/MS and allowed the selection of endogenous CDP-choline as a promising candidate biomarker for hypoxia-derived brain damage assessing intensity of retinal hypoxia. Results from this study will foster further research on CDP-choline changes occurring during resuscitation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn*
  • Biomarkers / metabolism
  • Chromatography, Liquid
  • Eye Proteins / metabolism*
  • Hypoxia / pathology*
  • Metabolomics*
  • Retina / pathology*
  • Swine
  • Tandem Mass Spectrometry

Substances

  • Biomarkers
  • Eye Proteins

Grants and funding

JE, JK and ITC acknowledge their personal grants (Sara Borrell CD11/00154, CD12/00667 and PFIS FI12/00109). AL acknowledges for a Miguel Servet grant (CP08/00125) and FISPI11/02942 grant from the Instituto Carlos III (Ministry of Economy and Competitiveness, Spain). MV acknowledges the FISPI11/0313 grant from the Instituto Carlos III. GQ acknowledges financial support from the Ministry of Economy and Competitiveness (SAF2012-39948). Financial support was also provided by the South-Eastern Norway Regional Health Authority. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.