Live cell imaging of cytoplasmic Ca2+ dynamics in Arabidopsis guard cells

Smrutisanjita Behera; Jörg Kudla

doi:10.1101/pdb.prot072983

Live cell imaging of cytoplasmic Ca2+ dynamics in Arabidopsis guard cells

Cold Spring Harb Protoc. 2013 Jul 1;2013(7):665-9. doi: 10.1101/pdb.prot072983.

Authors

Smrutisanjita Behera¹, Jörg Kudla

Affiliation

¹ Institute of Plant Biology and Biotechnology, University of Münster, 48149 Münster, Germany.

PMID: 23818665
DOI: 10.1101/pdb.prot072983

Abstract

This protocol describes a classical method for measuring cytoplasmic Ca(2+) dynamics in Arabidopsis thaliana guard cells. The Förster (fluorescence) resonance energy transfer (FRET)-based genetically modified Ca(2+) indicator Yellow Cameleon YC3.6, under the control of the guard cell-specific promoter GC1, is used for Ca(2+) measurements.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis / chemistry*
Calcium / analysis*
Calcium-Binding Proteins / analysis
Calcium-Binding Proteins / genetics
Cations, Divalent / analysis
Cytoplasm / chemistry*
Genes, Reporter
Image Processing, Computer-Assisted / methods*
Microscopy / methods*
Staining and Labeling / methods

Substances

Calcium-Binding Proteins
Cations, Divalent
yellow cameleon
Calcium