Shrimp hemocytes release extracellular traps that kill bacteria

Abstract

Extracellular traps (ETs) are formed from the DNA, histones and cytoplasmic antimicrobial proteins that are released from a range of vertebrate immune-cells in response to pathogenic stimulation. This novel defense mechanism has not been demonstrated in invertebrates. In this study, we investigated the formation of ETs in the crustacean Litopenaeus vannamei. We found that stimulation of shrimp hemocytes with phorbol myristate acetate (PMA), lipopolysaccharide (LPS) and live Escherichia coli all led to the formation of the characteristic ET fibers made from host cell DNA. After E. coli stimulation, we found that histone proteins were co-localized with these extracellular DNA fibers. The results further showed that E. coli were trapped by these ET-like fibers and that some of the trapped bacteria were permeabilized. All of these results are characteristic of the ETs that are seen in vertebrates and we therefore conclude that shrimp are also capable of forming extracellular traps.

Keywords: Extracellular traps; Hemocytes; Shrimp.