Skeletal muscle stem cells

Methods Mol Biol. 2013:1036:19-32. doi: 10.1007/978-1-62703-511-8_2.

Abstract

Skeletal muscle satellite cells (myoblasts) are the primary stem cells of skeletal muscle which contribute to growth, maintenance, and repair of the muscles. Satellite cells are the first stem cells used for cellular cardiomyoplasty more than 20 years ago. The isolation, culture, labeling, and identification of satellite cells are described in detail here. The implantation and outcomes of cellular cardiomyoplasty using satellite cells have been summarized in the previous chapter (Chapter 1).

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cardiomyoplasty / methods*
  • Cell Differentiation
  • Cell Separation / methods*
  • Cells, Cultured
  • Humans
  • In Situ Hybridization, Fluorescence
  • Muscle, Skeletal / cytology*
  • Muscle, Skeletal / physiology
  • Regeneration
  • Satellite Cells, Skeletal Muscle / cytology*
  • Satellite Cells, Skeletal Muscle / physiology