Objective: To develop a novel quantitative method for simultaneous analysis of 11 polyphenols in ratafee by reversed-phase high performance liquid chromatography.
Methods: The separation was performed on a Agilent Eclipse XDB C18 (4.6 mmx250 mm, 51 m) and the column temperature was set at 30 degrees C. The mobile phase was consisted of 0. 1% phosphoric acid (A) and methanol (B), with a linear gradient elution at a flow rate of I. 0 ml/min. The gradient program was as follow: 10% B isocratic (0-10 min), 10%-20% B liner (10-15 min), 20% B isocratic (15-25 min), 20%-60% B liner (25-65 min), 60% B isocratic (65-75 min). And detection wavelength was 210, 270, 320, 327 and 360 nm.
Results: The correlation coefficients of the 11 calibration curves were all higher than 0.9995. The scopes of the recovery rate was within the range of 85.1% to 112.0%, with RSDs no more than 2%. The limit of quantification (LOQ) and the detection limit (LOD) were 0.32-1.59 ng and 0.10-0.48 ng, respectively.
Conclusion: The method is accurate and reliable, and can successfully analyze different ratafee samples from different sources.