Objective: To study the effect of wheat oligo-peptides on antioxidant function in vitro and in vivo.
Methods: Male KM aged mice were randomly divided into 4 groups according to the serum MDA level, i. e. the negative control group, low-dose group, middle-dose group and high-dose group (0, 500, 1000, 2000 mg/kg BW of wheat oligo-peptides). After a 30-day administration of wheat oligo-peptides, the content of serum MDA, activity of SOD, GSH-Px and T-AOC were determined. The reducing power, DPPH radical and hydroxyl radical scavenging activity were also determined to evaluate the antioxidant activity in vitro.
Results: The wheat oligo-peptides were found a good reducing power in vitro (R=0.97), scavenging activity of DPPH radical and hydroxyl radical (RDPPH.=0.90, R.OH=0.91). In vivo experiment, the activity of serum SOD and GSH-Px, and the serum T-AOC of all treated groups was significantly increased compared with negative control group (P<0.05).
Conclusion: The results from this study showed that wheat oligo-peptides have a good antioxidant activity both in vitro and in vivo.