A Single Nucleotide Polymorphism within the Interferon Gamma Receptor 2 Gene Perfectly Coincides with Polledness in Holstein Cattle

PLoS One. 2013 Jun 21;8(6):e67992. doi: 10.1371/journal.pone.0067992. Print 2013.

Abstract

Polledness is a high impact trait in modern milk and beef production to meet the demands of animal welfare and work safety. Previous studies have mapped the polled-locus to the proximal region of the bovine chromosome 1 (BTA1) and narrowed it down to approximately 1 Mb. Sequencing of the positional candidate genes within the 1 Mb polled region and whole genome sequencing of Holsteins revealed a single nucleotide polymorphism (SNP) AC000158: g.1390292G>A within intron 3 of the interferon gamma receptor 2 gene (IFNGR2) in perfect co-segregation with polledness in Holsteins. This complete association was validated in 443 animals of the same breed. This SNP allows reliable genotyping of horned, heterozygous and homozygous polled Holsteins, even in animals that could not be resolved using the previously published haplotype for Holstein.

MeSH terms

  • Alleles
  • Animals
  • Cattle
  • DNA / chemistry
  • DNA / isolation & purification
  • DNA / metabolism
  • DNA Mutational Analysis
  • Female
  • Genotype
  • Haplotypes
  • Heterozygote
  • High-Throughput Nucleotide Sequencing
  • Homozygote
  • Interferon gamma Receptor
  • Linkage Disequilibrium
  • Phenotype
  • Polymorphism, Single Nucleotide*
  • Receptors, Interferon / genetics*

Substances

  • Receptors, Interferon
  • DNA

Grants and funding

This work has been funded by the German Research Community (Deutsche Forschungsgemeinschaft, DFG), Bonn, Germany (DI333/15-2). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.