Activity of lipase and chitinase immobilized on superparamagnetic particles in a rotational magnetic field

PLoS One. 2013 Jun 14;8(6):e66528. doi: 10.1371/journal.pone.0066528. Print 2013.

Abstract

We immobilize hydrolases such as lipase and chitinase on superparamagnetic particles, which are subjected to a rotational magnetic field, and measure the activities of the enzymes. We find that the activities of lipase and chitinase increase in the rotational magnetic field compared to those in the absence of a magnetic field and reach maximum at certain frequencies. The present methodology may well be utilized for the design and development of efficient micro reactors and micro total analysis systems (μ-TASs).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Candida albicans / enzymology
  • Chitinases / chemistry*
  • Enzymes, Immobilized / chemistry*
  • Fungal Proteins / chemistry*
  • Lipase / chemistry*
  • Magnetic Fields
  • Nanoparticles / chemistry*
  • Trichoderma / enzymology

Substances

  • Enzymes, Immobilized
  • Fungal Proteins
  • Lipase
  • Chitinases

Grants and funding

Grant for the Program for the Strategic Research Foundation at Private Universities S1101017 organized by the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.