miR-146b-5p inhibits glioma migration and invasion by targeting MMP16

Cancer Lett. 2013 Oct 10;339(2):260-9. doi: 10.1016/j.canlet.2013.06.018. Epub 2013 Jun 22.

Abstract

miR-146b-5p is frequently down-regulated in solid tumours, including prostate cancer, pancreatic cancer, and glioblastoma. However, the tumour-suppressive effects of miR-146b-5p in malignant gliomas have not been investigated thoroughly. Here, we found that decreased miR-146b-5p expression was strongly correlated with chromosome 10q loss in gliomas, especially glioblastomas. The overexpression of miR-146b-5p in glioblastoma cell lines led to MMP16 mRNA silencing, MMP2 inactivation, and the inhibition of tumour cell migration and invasion. Our results suggest that the restoration of miR-146b-5p expression may be a feasible approach for inhibiting the migration and invasion of malignant gliomas.

Keywords: Gliomas; Invasion; MMP16; Migration; miR-146b-5p.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cell Line, Tumor
  • Cell Movement / genetics*
  • Chromosome Deletion
  • Chromosomes, Human, Pair 10
  • Gelatinases / metabolism
  • Gene Expression
  • Gene Knockdown Techniques
  • Glioma / genetics*
  • Glioma / pathology*
  • Humans
  • Matrix Metalloproteinase 16 / chemistry
  • Matrix Metalloproteinase 16 / genetics*
  • Matrix Metalloproteinase 16 / metabolism
  • MicroRNAs / chemistry
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Neoplasm Invasiveness

Substances

  • MIRN146 microRNA, human
  • MicroRNAs
  • Gelatinases
  • Matrix Metalloproteinase 16