Fluorescent nanoparticle delivered dsRNA toward genetic control of insect pests

Adv Mater. 2013 Sep 6;25(33):4580-4. doi: 10.1002/adma.201301201. Epub 2013 Jun 24.

Abstract

A fluorescent cationic core-shell nanoparticle efficiently enters into cells with high transfection efficacy. A FNP/CHT10-dsRNA complex is orally fed to insect pests and knocks down a midgut-specific chitinase gene of the Asian corn borer, which leads to death. This is the first report on the genetic control of insect pests through a non-viral gene delivery system to knock down key developmental gene expression.

Keywords: dsRNAi; fluorescence; gene carrier; insect; nanoparticle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / drug effects
  • Cell Survival / drug effects
  • Dose-Response Relationship, Drug
  • Drosophila / cytology
  • Drosophila / drug effects*
  • Drosophila / genetics
  • Fluorescent Dyes / chemistry*
  • Genetic Vectors / chemistry*
  • Insect Control
  • Insecticides / pharmacology*
  • Molecular Structure
  • Nanoparticles / chemistry*
  • RNA, Double-Stranded / chemistry*
  • RNA, Double-Stranded / genetics
  • Structure-Activity Relationship

Substances

  • Fluorescent Dyes
  • Insecticides
  • RNA, Double-Stranded