A comment on the localization of cyanine dye binding to brush-border membranes by the fluorescence quenching of n-(9-anthroyloxy) fatty acid probes

Biochim Biophys Acta. 1990 Jul 9;1026(1):133-4. doi: 10.1016/0005-2736(90)90343-m.

Abstract

This work comments on the location and orientation of 3,3'-dipropylthiodicarbocyanine (diS-C3-(5)) in renal brush-border membrane vesicles (RBBMV) (Cabrini, G. and Verkman, A.S. (1986) Biochim. Biophys. Acta 862, 285-293) evaluated from collisional quenching of n-(9-anthroyloxy)stearic acid (n-AS) fluorescence. At variance with these authors, it is concluded that the quenching is due to resonance energy transfer. It is also shown that the fluorescence data are not clear evidence for the reported monomer and dimer locations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzothiazoles
  • Carbocyanines / metabolism*
  • Chemical Phenomena
  • Chemistry, Physical
  • Energy Transfer
  • Fluorescence
  • Fluorescent Dyes
  • Kidney / ultrastructure*
  • Liposomes / metabolism
  • Microvilli / metabolism*
  • Quinolines / metabolism*
  • Stearic Acids*

Substances

  • Benzothiazoles
  • Carbocyanines
  • Fluorescent Dyes
  • Liposomes
  • Quinolines
  • Stearic Acids
  • 3,3'-dipropyl-2,2'-thiadicarbocyanine
  • 9-(9-anthroyloxy)stearic acid
  • 2-(9-anthroyloxy)stearic acid