Immobilization of actively thromboresistant assemblies on sterile blood-contacting surfaces

Abstract

Rapid one-step modification of thrombomodulin with alkylamine derivatives such as azide, biotin, and PEG is achieved using an evolved sortase (eSrtA) mutant. The feasibility of a point-of-care scheme is demonstrated herein to site-specifically immobilize azido-thrombomodulin on sterilized commercial ePTFE vascular grafts, which exhibit superior thromboresistance compared with commercial heparin-coated grafts in a primate model of acute graft thrombosis.

Keywords: antifouling; biocompatible materials; biomedical applications; thrombomodulin; thrombosis.