Unidirectional migration of single smooth muscle cells under the synergetic effects of gradient swelling cue and parallel groove patterns

Lulu Han; Zhengwei Mao; Jindan Wu; Yang Guo; Tanchen Ren; Changyou Gao

doi:10.1016/j.colsurfb.2013.05.011

Unidirectional migration of single smooth muscle cells under the synergetic effects of gradient swelling cue and parallel groove patterns

Colloids Surf B Biointerfaces. 2013 Nov 1:111:1-6. doi: 10.1016/j.colsurfb.2013.05.011. Epub 2013 May 21.

Authors

Lulu Han¹, Zhengwei Mao², Jindan Wu¹, Yang Guo¹, Tanchen Ren¹, Changyou Gao³

Affiliations

¹ MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
² MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. Electronic address: zwmao@zju.edu.cn.
³ MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. Electronic address: cygao@mail.hz.zj.cn.

PMID: 23774121
DOI: 10.1016/j.colsurfb.2013.05.011

Abstract

The air-plasma treated poly(dimethylsiloxane) membrane with linear grooves and ridges is alternately assembled with poly(sodium 4-styrenesulfonate) (PSS)/poly(diallyldimethylammonium) chloride (PDADMAC) multilayers, which is then treated in a gradient solution of 3-5M NaCl, with the pattern direction parallel to the gradient. The synergetic effects of the surface topography and swelling gradient can effectively guide the unidirectional migration of single smooth muscle cells without impairment of their migration rate.

Keywords: Cell migration; Direction; Gradient; Groove patterns; Polyelectrolyte multilayers.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Movement* / drug effects
Cytoskeleton / drug effects
Electrolytes / pharmacology
Humans
Myocytes, Smooth Muscle / cytology*
Myocytes, Smooth Muscle / drug effects
Polyethylenes / pharmacology
Quaternary Ammonium Compounds / pharmacology
Surface Properties
Time-Lapse Imaging

Substances

Electrolytes
Polyethylenes
Quaternary Ammonium Compounds
poly-N,N-dimethyl-N,N-diallylammonium chloride