A simple, fast and highly sensitive assay for the detection of telomerase activity

Chem Commun (Camb). 2013 Jul 28;49(59):6596-8. doi: 10.1039/c3cc42571a.

Abstract

A highly sensitive and rapid PCR-free telomerase activity assay has been developed that uses SYBR Green intercalation into the G-quadruplex structures in the presence of K(+).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anthraquinones / pharmacology
  • Benzothiazoles
  • Cell Line
  • Diamines
  • G-Quadruplexes
  • HEK293 Cells
  • HeLa Cells
  • Hep G2 Cells
  • Humans
  • Organic Chemicals / chemistry
  • Organic Chemicals / metabolism
  • Potassium / chemistry
  • Quinolines
  • Structure-Activity Relationship
  • Telomerase / antagonists & inhibitors
  • Telomerase / chemistry
  • Telomerase / metabolism*

Substances

  • Anthraquinones
  • Benzothiazoles
  • Diamines
  • Organic Chemicals
  • Quinolines
  • SYBR Green I
  • 2,6-diaminoanthraquinone
  • Telomerase
  • Potassium