Exacerbation of experimental autoimmune encephalomyelitis by passive transfer of IgG antibodies from a multiple sclerosis patient responsive to immunoadsorption

Abstract

The pathogenic role of antibodies in multiple sclerosis (MS) is still controversial. We transferred to mice with experimental autoimmune encephalomyelitis (EAE), animal model of MS, IgG antibodies purified from a MS patient presenting a dramatic clinical improvement during relapse after selective IgG removal with immunoadsorption. Passive transfer of patient's IgG exacerbated motor paralysis and increased mouse central nervous system (CNS) inflammation and demyelination. Binding of patient's IgG was demonstrated in mouse CNS, with a diffuse staining of white matter oligodendrocytes. These data support a growing body of evidence that antibodies can play an important role in the pathobiology of MS.

Keywords: Antibodies; Demyelination; EAE; Experimental autoimmune encephalomyelitis; Immunoadsorption; LNCs; MS; Multiple sclerosis; PBS; PLP; Plasma exchange; T helper; TPE; Th; experimental autoimmune encephalomyelitis; lymph node cells; multiple sclerosis; myelin proteolipid protein; phosphate buffer saline; plasma exchange.