Abstract
Epigenetic manipulation of gene expression in Chaetomium indicum using a HDAC inhibitor led to the isolation of structurally diverse chaetophenols, and 3, 4 and 5 bear unprecedented polycyclic skeletons. The expression of two silent genes (pksCH-1 and pksCH-2) for nonreducing PKSs involved in chaetophenol biosynthesis was associated with an increase of histone acetylation level. The heterologous gene expression study in Aspergillus oryzae revealed pksCH-2 to be the NR-PKS gene for 8.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylation
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Aspergillus oryzae / chemistry*
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Aspergillus oryzae / genetics
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Benzopyrans / chemical synthesis*
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Benzopyrans / chemistry
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Chaetomium / chemistry*
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Cyclization
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Epigenomics
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Gene Expression Regulation, Fungal
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Genes, Fungal / genetics
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Molecular Structure
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Phenols / chemical synthesis*
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Phenols / chemistry*
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Polycyclic Compounds / chemical synthesis*
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Polycyclic Compounds / chemistry*
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Polyketides / chemical synthesis*
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Polyketides / chemistry
Substances
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Benzopyrans
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Phenols
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Polycyclic Compounds
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Polyketides