DNA origami as a DNA repair nanosensor at the single-molecule level

Angew Chem Int Ed Engl. 2013 Jul 22;52(30):7747-50. doi: 10.1002/anie.201301293. Epub 2013 Jun 13.

Abstract

The folding of DNA molecules by DNA origami is used in a nanosensor to analyze enzymatic DNA repair activity of hAGT. The method uses conformational changes that condition α-thrombin interaction with DNA aptamers, and illustrates the use of DNA origami as a proteinrecognition biosensor.

Keywords: DNA origami; DNA repair; G-quadruplexes; biosensors; hAGT.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiotensinogen / metabolism*
  • Aptamers, Nucleotide* / chemistry
  • Biosensing Techniques*
  • DNA Repair*
  • Humans
  • Microscopy, Atomic Force
  • Nanostructures* / chemistry
  • Nanotechnology*
  • Thrombin / metabolism*

Substances

  • AGT protein, human
  • Aptamers, Nucleotide
  • Angiotensinogen
  • Thrombin