BSA and fibrinogen adsorption on chitosan/κ-carrageenan polyelectrolyte complexes

Macromol Biosci. 2013 Aug;13(8):1072-83. doi: 10.1002/mabi.201200482. Epub 2013 Jun 14.

Abstract

PECs of chitosan/κ-carrageenan are prepared in three different volumetric rations. The complex formation is characterized in order to evaluate the blending formation. Blood compatibility is evaluated by protein adsorption (BSA and fibrinogen) and PEC toxicities are determined with fibroblast cell viability and proliferation. The swelling degree of PECs decreases when the amount of chitosan increases. Due to the linked film formation, PECs decrease BSA adsorption and increase fibrinogen adsorption when compared to the pristine chitosan and κ-carrageenan films. Although pristine chitosan and κ-carrageenan films produced similar cell expansion and viability, the PEC 50:50 vol% chitosan/κ-carrageenan PEC may be acceptable as a new scaffold for cell therapies, due to their effect on cell survival.

Keywords: cell proliferation; chitosan; polyelectrolyte complex; protein adsorption; κ-carrageenan.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Adsorption
  • Animals
  • Biocompatible Materials / chemical synthesis
  • Biocompatible Materials / metabolism*
  • Carrageenan / metabolism*
  • Cell Line
  • Cell Proliferation
  • Cell Survival
  • Cell- and Tissue-Based Therapy / methods
  • Chitosan / metabolism*
  • Electrolytes / chemical synthesis
  • Fibrinogen / metabolism*
  • Fibroblasts / metabolism
  • Mice
  • Microscopy, Atomic Force
  • Serum Albumin, Bovine / metabolism*
  • Surface Properties
  • Tissue Scaffolds

Substances

  • Biocompatible Materials
  • Electrolytes
  • Serum Albumin, Bovine
  • Carrageenan
  • Fibrinogen
  • Chitosan