A novel method for the analysis of 3-mercaptopyruvate using high-performance liquid chromatography with fluorescence detection

J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Jul 15:931:56-60. doi: 10.1016/j.jchromb.2013.05.011. Epub 2013 May 22.

Abstract

3-Mercaptopyruvate (3-MP) is a metabolite of cysteine present in mammalian tissues and is known to be a substrate of 3-mercaptopyruvate sulfurtransferase (3MST, EC.3.4.1.2). The physiological relevance of the 3-MP pathway has not been fully recognized because the metabolic behavior of 3-MP remains unclear. Here, we describe a novel method using high-performance liquid chromatography with fluorescence detection to measure 3-MP formation from cysteine. To demonstrate the practical value of the present method, we applied it to analyze the 3-MP produced in biological samples from mouse tissue.

MeSH terms

  • Animals
  • Brain / metabolism
  • Brain Chemistry
  • Chromatography, High Pressure Liquid / methods*
  • Cysteine / analogs & derivatives*
  • Cysteine / analysis
  • Cysteine / metabolism
  • Ketoglutaric Acids / metabolism
  • Liver / chemistry
  • Liver / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spectrometry, Fluorescence
  • Transaminases

Substances

  • Ketoglutaric Acids
  • 3-mercaptopyruvic acid
  • Transaminases
  • cysteine aminotransferase
  • Cysteine