Abstract
Five new pregnane-type steroids, sclerosteroids J-N (1-5), and a diterpenoid with a new chemotype 3-methyl-5-(10'-acetoxy-2',6',10'-trimethylundecyl)-2-penten-5-olide (6), have been isolated from a soft coral Scleronephthya gracillimum. The structures of the metabolites were determined by extensive spectroscopic analysis. Compound 4 exhibited cytotoxicity against HepG2, A549, and MDA-MB-231 cancer cell lines. Furthermore, steroids 2 and 4 were found to significantly inhibit the accumulation of the pro-inflammatory iNOS protein, and 1, 2, 4 and 5 could effectively reduce the accumulation of COX-2 protein in LPS-stimulated RAW264.7 macrophage cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anthozoa / metabolism*
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Anti-Inflammatory Agents / chemistry
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Anti-Inflammatory Agents / isolation & purification
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Anti-Inflammatory Agents / pharmacology*
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Antineoplastic Agents / chemistry
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Antineoplastic Agents / isolation & purification
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Antineoplastic Agents / pharmacology*
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Cell Line
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Cell Line, Tumor
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Cyclooxygenase 2 / drug effects
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Cyclooxygenase 2 / metabolism
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Diterpenes / chemistry
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Diterpenes / isolation & purification
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Diterpenes / pharmacology
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Hep G2 Cells
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Humans
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Inflammation / drug therapy
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Inflammation / pathology
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Lipopolysaccharides
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Macrophages / drug effects
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Macrophages / metabolism
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Mice
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Neoplasms / drug therapy
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Neoplasms / pathology
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Nitric Oxide Synthase Type II
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Spectrum Analysis
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Steroids / chemistry
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Steroids / isolation & purification
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Steroids / pharmacology
Substances
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Anti-Inflammatory Agents
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Antineoplastic Agents
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Diterpenes
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Lipopolysaccharides
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Steroids
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Nitric Oxide Synthase Type II
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Cyclooxygenase 2