Analysis of microarrays by MALDI-TOF MS

Angew Chem Int Ed Engl. 2013 Jul 15;52(29):7477-81. doi: 10.1002/anie.201302455. Epub 2013 Jun 11.

Abstract

Ligand libraries can be printed onto a sandwich composed of activated lipids embedded in a hydrophobic layer conjugated to an indium-tin oxide (ITO) surface. Arrays produced this way can be analyzed by fluorescence spectroscopy and mass spectrometry. Applications include the assignment of enzyme specificity, the profiling of glycoforms and the identification of lectins.

Keywords: lectins; mass spectrometry; microarrays; proteomics; transferases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Lectins / analysis
  • Lipids / chemistry
  • Protein Array Analysis
  • Proteomics
  • Spectrometry, Fluorescence
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*
  • Surface Properties
  • Tin Compounds / chemistry
  • Transferases / metabolism

Substances

  • Lectins
  • Lipids
  • Tin Compounds
  • indium tin oxide
  • Transferases