Optical detection of epigenetic marks: sensitive quantification and direct imaging of individual hydroxymethylcytosine bases

Chem Commun (Camb). 2013 Oct 7;49(77):8599-601. doi: 10.1039/c3cc42543f.

Abstract

Herein we report the specific labelling of the epigenetic modification 5-hydroxymethyl-cytosine along genomic DNA molecules with a fluorescent reporter molecule. Enzymatic glucosylation followed by a click chemistry reaction enables single molecule detection as well as global quantification of 5hmC in genomic DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5-Methylcytosine / analogs & derivatives
  • Animals
  • Bacteriophage lambda / chemistry
  • Cytosine / analogs & derivatives*
  • Cytosine / analysis
  • DNA / chemistry*
  • DNA Methylation*
  • Epigenesis, Genetic
  • Fluorescent Dyes / analysis
  • Mice
  • Models, Molecular
  • Optical Imaging*

Substances

  • Fluorescent Dyes
  • 5-hydroxymethylcytosine
  • 5-Methylcytosine
  • Cytosine
  • DNA