Abstract
Herein we report the specific labelling of the epigenetic modification 5-hydroxymethyl-cytosine along genomic DNA molecules with a fluorescent reporter molecule. Enzymatic glucosylation followed by a click chemistry reaction enables single molecule detection as well as global quantification of 5hmC in genomic DNA.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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5-Methylcytosine / analogs & derivatives
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Animals
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Bacteriophage lambda / chemistry
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Cytosine / analogs & derivatives*
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Cytosine / analysis
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DNA / chemistry*
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DNA Methylation*
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Epigenesis, Genetic
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Fluorescent Dyes / analysis
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Mice
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Models, Molecular
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Optical Imaging*
Substances
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Fluorescent Dyes
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5-hydroxymethylcytosine
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5-Methylcytosine
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Cytosine
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DNA