Graphene has attracted a great deal of scientific interest due to its unique properties and its various prospective applications. However, the use of graphene in technological purposes depends on the development of suitable methodologies for its mass production. Several environmentally safe methods have been emerging for the production of graphene from graphite oxide which are based on the solvothermal and the electrochemical processes. Herein, we developed a green, simple and cost effective approach to produce graphene by using an Escherichia fergusoni as a reducing agent. This reduction method avoids the use of environmentally harmful reagents such as hydrazine and hydrazine hydrate. The resulting reduced graphene oxide (rGO) was characterized by a wide range of analytical techniques. UV-Vis spectra of the samples confirm the transition of graphene oxide (GO) into graphene. Fourier transform infra-red spectroscopy (FT-IR) spectrum of the rGO indicates a significant reduction of the intensity of all oxygen containing moieties suggesting efficient conversion of GO to rGO. X-ray diffraction (XRD) was used to investigate the crystalline of graphene nanosheets, whereas high resolution scanning electron microscopy (SEM) and atomic force microscopy (AFM) were employed to investigate the morphologies of prepared graphene. Raman spectroscopy images provide clear evidence for the formation of graphene. All these findings clearly indicate that GO could be reduced by simple, eco-friendly method by using E. fergusoni to produce water dispersible graphene.