There are various methods and techniques that can be utilize for abdominal biopsy. Some of these techniques have been utilized for over four decades. However, there are different variations to these techniques that have been recently developed to increase tissue retrieval and decrease complications. This manuscript will review a number of different modifications of prior CT or ultrasound techniques utilized with either CT or ultrasound that are helpful to be more effective in tissue retrieval. Newer guidance systems including the use of contrast-enhanced sonography and multi-modality image fusion biopsy guidance are reviewed. These different techniques can increase tissues retrieval, increase accuracy of needle placement, decrease the time for the procedure, and minimize complications.