Self-assembly of DNA-based drug delivery nanocarriers with rolling circle amplification

Methods. 2014 May 15;67(2):198-204. doi: 10.1016/j.ymeth.2013.05.024. Epub 2013 Jun 7.

Abstract

DNA nanostructures have recently emerged as a type of drug delivery nanocarriers due to their suitable sizes, well-defined structures and low-toxicity. Here, we present a protocol for the assembly of DNA nanoribbon structures with rolling circle amplification (RCA) and delivery of CpG oligonucleotide. DNA nanoribbons with different dimensions and patterns were assembled with long RCA strands and several short staples. Significantly, we demonstrated they exhibited high-efficiency cellular uptake and improved immunostimulatory activity compared with ss- or ds- DNA.

Keywords: DNA nanoribbons; Intracellular delivery; Nucleic acids; Rolling circle amplification.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / chemistry
  • Adjuvants, Immunologic / pharmacology
  • Animals
  • Bacteriophage M13 / genetics
  • Base Sequence
  • Cell Line
  • CpG Islands
  • DNA, Single-Stranded / chemistry*
  • DNA, Viral / chemistry
  • Drug Carriers / chemistry*
  • Drug Carriers / pharmacology
  • Mice
  • Nucleic Acid Amplification Techniques
  • Nucleic Acid Conformation
  • Oligodeoxyribonucleotides / chemistry
  • Oligodeoxyribonucleotides / pharmacology
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Adjuvants, Immunologic
  • DNA, Single-Stranded
  • DNA, Viral
  • Drug Carriers
  • Oligodeoxyribonucleotides
  • Tumor Necrosis Factor-alpha