Abstract
The study investigated the effect of taurine on cell viability and neurotrophic gene expression in arsenite-treated human neuroblastoma SH-SY5Y cells. Arsenite-induced intracellular reactive oxygen species (ROS) and interrupted cell cycle in SH-SY5Y cells. In addition, arsenite reduced mitochondria membrane potential (MMP) and decreased neurotrophic gene expressions such as n-myc downstream-regulated gene 4 (NDRG-4), brain-derived neurotrophic factor (BDNF) and sirtuin-1 (SIRT-1) in SH-SY5Y cells. In parallel, taurine prevented cell cycle, restored MMP and reduced the intracellular ROS level, and taurine recovered NDRG-4, BDNF and SIRT-1 gene expressions in arsenite-treated SH-SY5Y cells while taurine alone has no effect on these parameters.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arsenites / pharmacology*
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Brain-Derived Neurotrophic Factor / genetics*
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Brain-Derived Neurotrophic Factor / metabolism
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Cell Cycle Checkpoints / drug effects*
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Cell Survival / drug effects
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Gene Expression Profiling
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Gene Expression Regulation / drug effects*
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Humans
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Membrane Potential, Mitochondrial / drug effects
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Muscle Proteins / genetics*
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Muscle Proteins / metabolism
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Nerve Tissue Proteins / genetics*
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Nerve Tissue Proteins / metabolism
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Reactive Oxygen Species / metabolism
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Real-Time Polymerase Chain Reaction
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Sirtuin 1 / genetics*
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Sirtuin 1 / metabolism
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Taurine / pharmacology*
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Tumor Cells, Cultured
Substances
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Arsenites
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Brain-Derived Neurotrophic Factor
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Muscle Proteins
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NDRG4 protein, human
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Nerve Tissue Proteins
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Reactive Oxygen Species
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Taurine
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BDNF protein, human
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SIRT1 protein, human
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Sirtuin 1
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arsenite