Structural elucidation of a novel water-soluble fructan isolated from Wedelia prostrata

Abstract

A new heteropolysaccharide, WPP60A, with a molecular weight of 2.4 kDa, was isolated from Wedelia prostrata by hot water extraction, and purified by DEAE Sepharose fast flow and Sephacryl S-200 high-resolution chromatography. Compositional analysis, and methylation analysis, combined with (1)H, (13)C NMR spectroscopy including 2D NMR (COSY, TOCSY, HMQC, NOESY, and HMBC) experiments demonstrated that WPP60A was composed of primarily fructose and low glucose. Results indicated that this new heteropolysaccharide consists of a repeating unit with the following structure: [Formula: see text].