Direct targeting of proteins to lipid droplets demonstrated by time-lapse live cell imaging

Torahiko Tanaka; Kazumichi Kuroda; Masanori Ikeda; Nobuyuki Kato; Kazufumi Shimizu; Makoto Makishima

doi:10.1016/j.jbiosc.2013.05.006

Direct targeting of proteins to lipid droplets demonstrated by time-lapse live cell imaging

J Biosci Bioeng. 2013 Nov;116(5):620-3. doi: 10.1016/j.jbiosc.2013.05.006. Epub 2013 Jun 4.

Authors

Torahiko Tanaka¹, Kazumichi Kuroda, Masanori Ikeda, Nobuyuki Kato, Kazufumi Shimizu, Makoto Makishima

Affiliation

¹ Division of Biochemistry, Department of Biomedical Sciences, Nihon University School of Medicine, 30-1 Oyaguchi-kamimachi, Itabashi-ku, Tokyo 173-8610, Japan. Electronic address: tanaka.torahiko@nihon-u.ac.jp.

PMID: 23742748
DOI: 10.1016/j.jbiosc.2013.05.006

Abstract

A protein that specifically targets lipid droplets (LDs) was created by connecting two domains of nonstructural protein 4B containing amphipathic helices from hepatitis C virus. We demonstrated its direct targeting and accumulation to the LD surface by time-lapse live cell imaging, comparable to those observed with adipose differentiation-related protein.

Keywords: Adipose differentiation-related protein; Hepatitis C virus; Lipid droplets; Nonstructural protein 4B; Time-lapse imaging.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Cell Survival
Hepacivirus / chemistry*
Humans
Lipids*
Membrane Proteins / metabolism
Microscopy, Confocal
Perilipin-2
Protein Structure, Tertiary
Proteins / chemistry*
Proteins / genetics
Proteins / metabolism*
Substrate Specificity
Time Factors
Viral Nonstructural Proteins / chemistry

Substances

Lipids
Membrane Proteins
NS4B protein, flavivirus
PLIN2 protein, human
Perilipin-2
Proteins
Viral Nonstructural Proteins