In this study, the water quality status of river Solani in India was assessed under the influence of urbanization. Physico-chemical and biological analysis of water reflected maximum adverse impact during summer low flow season. Variation in river flows during monsoon, post monsoon and low flow seasons was found to substantially affect the river water quality regime. Whereas the monsoon season displayed addition of suspended impurities and provided dilution in the dissolved components, the summer low flow season revealed an anaerobic condition in the river as the entire river flow comprised of only drain effluents. All the drains were observed to carry contaminated water with impurities from various point and non-point sources emanating from diverse human activities. The present study indicated that the drain waste water deserves a prior treatment in order to protect the Solani river water from pollution.