Single layer centrifugation with androcoll-p can be scaled-up to process larger volumes of boar semen

Marjet van Wienen; Anders Johannisson; Margareta Wallgren; Joyce Parlevliet; Jane M Morrell

doi:10.5402/2011/548385

Single layer centrifugation with androcoll-p can be scaled-up to process larger volumes of boar semen

ISRN Vet Sci. 2010 Nov 29:2011:548385. doi: 10.5402/2011/548385. Print 2011.

Authors

Marjet van Wienen¹, Anders Johannisson, Margareta Wallgren, Joyce Parlevliet, Jane M Morrell

Affiliation

¹ Division of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences-SLU, P. O. Box 7054, 75007 Uppsala, Sweden ; Faculty of Veterinary Medicine, 3508TD Utrecht, The Netherlands.

Abstract

The objective of this study was to scale-up the procedure for Single Layer Centrifugation (SLC) through Androcoll(TM)-P, as a preliminary step towords processing the whole ejaculate. The first experiment compared Single Layer Centrifugation using 4.5 mL and 15 mL extended ejaculate (SLC-4.5 and SLC-15, resp.), assessing sperm quality by objective motility analysis, morphology, viability, and the production of reactive oxygen species (ROS). In the second experiment, SLC-4.5 was compared to Single Layer Centrifugation with 25 mL extended ejaculate (SLC-25) using motility analysis and morphology. In both experiments, normal morphology and linear motility were significantly higher in the SLC-selected samples than in the uncentrifuged controls (P < .001), whereas total motility and membrane integrity were unchanged. Although ROS production was higher in the SLC-selected samples than in the controls (P < .01), this might have been due to the presence of antioxidants in seminal plasma in the latter. In conclusion, there was no difference in sperm quality between SLC-4.5 and SLC-15 samples, or between SLC-4.5 and SLC-25 samples, indicating that the SLC method can be scaled-up successfully.