Phenotypic and genotypic characterization of acyclovir-resistant corneal HSV-1 isolates from immunocompetent patients with recurrent herpetic keratitis

J Clin Virol. 2013 Sep;58(1):321-4. doi: 10.1016/j.jcv.2013.05.001. Epub 2013 May 31.

Abstract

Herpes simplex virus type 1 (HSV-1) is a leading cause of corneal blindness. Acyclovir (ACV) constitutes the standard treatment of HSV infections including herpetic keratitis (HK). HSV resistance to ACV is mainly described in immunocompromised patients. We describe two cases of ACV-resistant corneal HSV-1 in immunocompetent individuals with recurrent HK.

Keywords: Herpetic keratitis; Immunocompetent patient; Phenotypic and genotypic antiviral resistance assay; Resistance to acyclovir.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acyclovir / pharmacology
  • Acyclovir / therapeutic use*
  • Aged
  • Aged, 80 and over
  • Amino Acid Substitution
  • Antiviral Agents / pharmacology
  • Antiviral Agents / therapeutic use*
  • DNA-Directed DNA Polymerase / genetics
  • Drug Resistance, Viral*
  • Exodeoxyribonucleases / genetics
  • Herpesvirus 1, Human / drug effects
  • Herpesvirus 1, Human / genetics*
  • Herpesvirus 1, Human / isolation & purification
  • Humans
  • Keratitis, Herpetic / drug therapy*
  • Keratitis, Herpetic / virology*
  • Male
  • Microbial Sensitivity Tests
  • Mutant Proteins / genetics
  • Mutation, Missense
  • Recurrence
  • Thymidine Kinase / genetics
  • Viral Proteins / genetics

Substances

  • Antiviral Agents
  • Mutant Proteins
  • Viral Proteins
  • Thymidine Kinase
  • DNA-Directed DNA Polymerase
  • Exodeoxyribonucleases
  • DNA polymerase, Simplexvirus
  • Acyclovir